The results obtained from the humidity, total ash, sulphated
ash, acid-insoluble ash and total alkaloid determination assays
are reported in Table 1
. During the course of our ongoing
studies on Turkish Galanthus species, previously Herba and
Bulbus Galanthi drugs prepared from G. elwesii
Ćelak., G. trojanus
A.P. Davis & N. Özhatay and G.
Bieb. subsp. byzantinus (Baker) D. A. Webb were
investigated for their contents of humidity, total ash, sulphated
ash and total alkaloids19-22
. Previous data and the results of
the present study, may be utilized for the determination of the
standard values for the elaboration of prospective monographs
on Herba and Bulbus Galanthi.
The total alkaloidal content is an important criterion for the
evaluation of the quality of Herba and Bulbus Galanthi. In our
study, the total alkaloids ranged between 0.247-0.499 %. Aerial
parts of G. woronowii collected from Derepazarı, Rize were
found to contain the maximal amount of total alkaloids. The
minimal amount of total alkaloids was detected in the aerial
parts of G. woronowii collected from Çaykara, Trabzon (Table
1). Previously, the contents of individiual alkaloids and the
quantity of the total bases obtained during isolation studies
have been reported for this plant10,23,24. However, to the
best of our knowledge, this is the first report on the
quantification of the total alkaloids in G. woronowii.
In addition to the quality control determinations,
acetylcholinesterase inhibitor potentials of the alkaloidal
extracts were screened by TLC in combination with bioactivity
staining based on Ellman's method. The active extracts were detected by the formation of white spots after spraying the
substrate, dye and enzyme which gave a yellow background17. All of the alkaloidal extracts contained galanthamine and
also other alkaloids with AchE inhibitory activity (Figure 1). It
is already known that AchE inhibitory activity is related
mainly with galanthamine- and lycorine-type Amaryllidaceae
alkaloids14,25,26. Our ongoing phytochemical studies on
this species, revealed that G. woronowii contained galanthamine,
lycorine and other alkaloids belonging to the galanthamine- or
lycorine-type of Amaryllidaceae alkaloids18,27,28 which
have been previously shown to possess AchE inhibitory
Click Here to Zoom
|FIGURE 1: AChE inhibitory activity of alkaloidal extracts of G. woronowii.
G: Galanthamine, 1: Bulbus/Derepazarı,Rize; 2: Herba/Derepazarı,Rize; 3: Bulbus/
Çaykara,Trabzon; 4: Herba/Çaykara,Trabzon.White spots indicate inhibition.
This study was financially supported by Ege University
Research Fund (No: 09/ECZ/08) and partially supported by
TUBITAK (No:104T272) and EBILTEM (No: 2007/BIL/007).